Integrated Nutrient Recovery and Decolorization from Textile Effluent in an Algal System

From Wastewater to Energy

Authors

  • Md Abul Hasanath Assistant Professor, Department of Civil Engineering, Dhaka International University, Bangladesh; Research Scholar, Department of Civil Engineering, Indian Institute of Technology Hyderabad, India
  • Md Mostafizur Rahman Assistant Professor, Department of Civil Engineering, Presidency University, Bangladesh
  • Md Jahid Hasan Deputy Director & Head, Admission and Public Relations, Presidency University, Bangladesh
  • Md Nashir Uddin PhD Researcher and Graduate TA, Department of Civil & Environmental Engineering, Morgan State University, United States of America
  • Monir Hossain PhD Researcher, Department of Civil & Environmental Engineering, Portland State University, United States of America https://orcid.org/0009-0007-2841-9574
  • Md Nazmul Hasan Postgraduate Student, Institute of Business Administration, Rajshahi University, Bangladesh
  • Azahar M. Mishkat PhD Researcher, School of Material Science and Engineering, Tongji University, Shanghai, China
  • Nahid Islam Undergraduate Student, Department of Civil Engineering, Military Institute of Science and Technology, Bangladesh

DOI:

https://doi.org/10.66460/puj.v4i1.7

Keywords:

Bioenergy, HRAP, Microalgae, Nutrient recovery

Abstract

This study investigated continuous textile wastewater treatment using a mixed microalgal consortium (Chlorella sp., Parachlorella sp., Coelastrella sp., and Scenedesmus sp.) in a 10-L high-rate algal pond (HRAP) system. In contrast to earlier investigations that focused on the optimization of specific operating variables under either batch or semi-continuous conditions, the current investigation attempted to optimize the HRT, OLR, and SRT simultaneously in a continuous mode with genuine textile wastewater, while at the same time conducting a downstream evaluation of biomass utilization for energy production. Textile wastewater with an initial COD of 1520 mg/L, BOD5 of 620 mg/L, and dye concentration measured at λmax= 580 nm was treated under varying HRT conditions (3-10 days). Optimal performance was achieved at HRT of 5 days and SRT of 20 days, resulting in 72% COD removal, 80% BOD5 removal, 88% TN removal, and 80% decolorization. All improvements in pollutant removal and biomass productivity at optimal HRT and SRT were statistically significant (p < 0.05, one-way ANOVA). Biomass productivity reached 56.3 mg/L.d with a balanced biochemical composition of 42.5% protein, 28.3% lipid, and 18.7% carbohydrate. Elemental analysis (C 47.2%, H 6.8%, N 8.1%, S 1%) supported theoretical energy estimates of 342 mL CH4/g VS for biomethane and 83-98 mg bioethanol/g biomass. The integrated system demonstrates a viable pathway for the textile sector to transition toward a sustainable circular bioeconomy, coupling effective effluent treatment with energy-rich biomass production.

References

Abdel-Raouf, N., Al-Homaidan, A. A., & Ibraheem, I. B. M. (2012). Microalgae and wastewater treatment. Saudi Journal of Biological Sciences, 19(3), 257–275. https://doi.org/10.1016/J.SJBS.2012.04.005

Acién Fernández, F. G., Gómez-Serrano, C., & Fernández-Sevilla, J. M. (2018). Recovery of Nutrients From Wastewaters Using Microalgae. Frontiers in Sustainable Food Systems, 2. https://doi.org/10.3389/fsufs.2018.00059

Anwar, T. Bin, Behrose, B., & Ahmed, S. (2018). Utilization of textile sludge and public health risk assessment in Bangladesh. Sustainable Environment Research, 28(5), 228–233. https://doi.org/10.1016/j.serj.2018.04.003

APHA, AWWA, & WEF. (2017). Standard methods for the examination of water and wastewater (R. B. Baird, A. D. Eaton, & E. W. Rice, Eds.; 23rd ed.). Washington, DC.

Aragaw, T. A., & Asmare, A. M. (2018). Phycoremediation of textile wastewater using indigenous microalgae. Water Practice and Technology, 13(2), 274–284. https://doi.org/10.2166/wpt.2018.037

Behera, D., Banerjee, S., & Chatterjee, P. (2025). CCD-RSM optimization of CO2, nitrate, and nanoparticle levels for enhanced biomass, lipid, and carbohydrate yields in Chlorella pyrenoidosa. Biomass Conversion and Biorefinery, 15(12), 18361–18374. https://doi.org/10.1007/s13399-025-06679-2

Behera, D., Fathima, J., Saady, N. M. C., Zendehboudi, S., Albayati, T. M., Al-nayili, A., Chatterjee, P., Ponnusami, V., Peach, B., & Espinoza, J. E. R. (2026). Sustainable agriculture through environmental adaptation engineering for waste management. Green Technologies and Sustainability, 4(1), 100242. https://doi.org/10.1016/j.grets.2025.100242

Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911–917. https://doi.org/10.1139/o59-099

Buswell, A. M., & Mueller, H. F. (1952). Mechanism of Methane Fermentation. Industrial & Engineering Chemistry, 44(3), 550–552. https://doi.org/10.1021/ie50507a033

de Godos, I., Arbib, Z., Lara, E., & Rogalla, F. (2016). Evaluation of High Rate Algae Ponds for treatment of anaerobically digested wastewater: Effect of CO2 addition and modification of dilution rate. Bioresource Technology, 220, 253–261. https://doi.org/10.1016/j.biortech.2016.08.056

El-Gawad, H. A., Ghaly, M. Y., El Hussieny, N. F., Abdel Kreem, M., & Reda, Y. (2024). Novel collector design and optimized photo-fenton model for sustainable industry textile wastewater treatment. Scientific Reports, 14(1), 8573. https://doi.org/10.1038/s41598-024-58610-w

Fazal, T., Rehman, M. S. U., Javed, F., Akhtar, M., Mushtaq, A., Hafeez, A., Alaud Din, A., Iqbal, J., Rashid, N., & Rehman, F. (2021). Integrating bioremediation of textile wastewater with biodiesel production using microalgae (Chlorella vulgaris). Chemosphere, 281, 130758. https://doi.org/10.1016/j.chemosphere.2021.130758

García, J., Mujeriego, R., & Hernández-Mariné, M. (2000). High rate algal pond operating strategies for urban wastewater nitrogen removal. Journal of Applied Phycology, 12(3–5), 331–339. https://doi.org/10.1023/A:1008146421368

Hasanath, M. A., & Chatterjee, P. (2025). Biochemical Methane Potential of Microalgae Cultivated in High-Strength Beverage Wastewater: Implications for Energy Enhancement. Journal of Hazardous, Toxic, and Radioactive Waste, 29(1), 1–9. https://doi.org/10.1061/JHTRBP.HZENG-1364

Hasanath, M. A., Ganesh, S., M Siddique, A., & Uddin, M. N. (2020). Assessment of beverage sludge to utilize as agricultural soil. In Imam, Rahman, & Pal (Eds.), Proceedings of the 5th International Conference on Advances in Civil Engineering (ICACE 2020) (pp. 254–260). Chittagong University of Engineering and Technlogy (CUET), Bangladesh. https://www.researchgate.net/publication/351524903

Hasanath, M. A., Panigrahi, A., Chaitanya, N. K., & Chatterjee, P. (2026). Treatment of beverage wastewater using microalgae: A focus on COD removal, biomass production, and lipid accumulation. Bioresource Technology Reports, 33, 102467. https://doi.org/10.1016/j.biteb.2025.102467

Hasanath, M. A., Uddin, M. N., & Ashraf, M. (2021). Fabrication of eco-friendly water purifier using RO and pedaling energy. In Imam, Rahman, & Pal (Eds.), Proceedings of the 5th International Conference on Advances in Civil Engineering (ICACE 2020) (pp. 29–4).

Herrera, J. S., & Moreno, Z. R. (2022). Biodegradation of Erionyl Turquoise azoic textile dye from Chlorella vulgaris microalgae in simulated wastewater. Journal of Textile Engineering & Fashion Technology, 8(4), 121–124. https://doi.org/10.15406/jteft.2022.08.00309

Jadhav, V., Dhanwate, Y., Raut, P., Shinde, S., Sawant, R., & Bhagare, A. (2025). Efficient photocatalytic methylene blue dye degradation from green-synthesized silver-doped iron oxide (Ag@Fe2O3) nanostructures. Discover Nano, 20(1), 66. https://doi.org/10.1186/s11671-025-04242-6

Kadam, R., Khanthong, K., Park, B., Jun, H., & Park, J. (2023). Realizable wastewater treatment process for carbon neutrality and energy sustainability: A review. Journal of Environmental Management, 328, 116927. https://doi.org/10.1016/j.jenvman.2022.116927

Li, W., Peng, S., Jiao, H., Zhang, J., Huang, Y., & Sun, L. (2021). Performance assessment of microalgal-based wastewater treatment (MBWT) system in response to operation modes, hydraulic retention time (HRT) and cyclical light. Desalination and Water Treatment, 229, 79–92. https://doi.org/10.5004/dwt.2021.27368

Loewus, F. A. (1952). Improvement in Anthrone Method for Determination of Carbohydrates. Analytical Chemistry, 24(1), 219. https://doi.org/10.1021/ac60061a050

Lowry, OliverH., Rosebrough, NiraJ., Farr, A. L., & Randall, RoseJ. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275. https://doi.org/10.1016/S0021-9258(19)52451-6

Mohamadnia, S., Thygesen, A., Ghofrani-Isfahani, P., Monachese, A. P., Valverde-Pérez, B., & Angelidaki, I. (2023). Valorization of potato starch wastewater using anaerobic acidification coupled with Chlorella sorokiniana cultivation. Journal of Applied Phycology, 35(6), 2645–2658. https://doi.org/10.1007/s10811-023-03046-4

Mohammed, K., Ahammad, S. Z., Sallis, P. J., & Mota, C. R. (2023). Hybrid microalgae-activated sludge system for carbon-efficient wastewater treatment. Water Science & Technology, 88(3), 586–594. https://doi.org/10.2166/wst.2023.246

Moody, J. W., McGinty, C. M., & Quinn, J. C. (2014). Global evaluation of biofuel potential from microalgae. Proceedings of the National Academy of Sciences, 111(23), 8691–8696. https://doi.org/10.1073/pnas.1321652111

Novoveská, L., Nielsen, S. L., Eroldoğan, O. T., Haznedaroglu, B. Z., Rinkevich, B., Fazi, S., Robbens, J., Vasquez, M., & Einarsson, H. (2023). Overview and Challenges of Large-Scale Cultivation of Photosynthetic Microalgae and Cyanobacteria. Marine Drugs, 21(8), 445. https://doi.org/10.3390/md21080445

Olabi, A. G., Shehata, N., Sayed, E. T., Rodriguez, C., Anyanwu, R. C., Russell, C., & Abdelkareem, M. A. (2023). Role of microalgae in achieving sustainable development goals and circular economy. Science of The Total Environment, 854, 158689. https://doi.org/10.1016/j.scitotenv.2022.158689

Pandey, A., Kant, G., Chaudhary, A., Amesho, K. T. T., Reddy, K., & Bux, F. (2024). Axenic green microalgae for the treatment of textile effluent and the production of biofuel: a promising sustainable approach. World Journal of Microbiology and Biotechnology, 40(3), 81. https://doi.org/10.1007/s11274-023-03863-2

Park, J. B. K., & Craggs, R. J. (2010). Wastewater treatment and algal production in high rate algal ponds with carbon dioxide addition. Water Science and Technology, 61(3), 633–639. https://doi.org/10.2166/wst.2010.951

Periyasamy, A. P. (2024). Recent Advances in the Remediation of Textile-Dye-Containing Wastewater: Prioritizing Human Health and Sustainable Wastewater Treatment. Sustainability, 16(2), 495. https://doi.org/10.3390/su16020495

Pham, L. A., Laurent, J., Bois, P., Teshome, T. M., & Wanko, A. (2021). Operating a semi-continuous raceway pond allows to link pH and oxygen dynamics to the interaction between microalgae and bacteria. Desalination and Water Treatment, 211, 105–116. https://doi.org/10.5004/dwt.2021.26506

Rahman, M. T., Saha, G. C., Hasanath, M. A., & Uddin, M. N. (2022). Potential Utilization of Textile Dyeing Sludge, Pet Granules, and Fly Ash in Lightweight Concrete Block. In S. Arthur, M. Saitoh, & S. K. Pal (Eds.), Advances in Civil Engineering: Lecture Notes in Civil Engineering, Select Proceedings of ICACE 2020 (Vol. 184, pp. 55–63). Springer. https://doi.org/10.1007/978-981-16-5547-0_6

Rani, S., Imran, M., Haider, A., Shahzadi, A., Ul‐Hamid, A., Somaily, H. H., Moeen, S., Khan, M., Nabgan, W., & Ikram, M. (2023). Dye Degradation, Antimicrobial Activity, and Molecular Docking Analysis of Samarium‐Grafted Carbon Nitride Doped‐Bismuth Oxobromide Quantum Dots. Global Challenges, 7(12). https://doi.org/10.1002/gch2.202300118

Saha, G. C., Hasanath, M. A., Uddin, M. N., & Hasan, M. (2022). Sustainable Utilization of Textile Dyeing Sludge and Coal Fly Ash by Brick Production Through Traditional Kilns. Nature Environment and Pollution Technology, 21(3), 971–980. https://doi.org/10.46488/NEPT.2022.v21i03.004

Suali, E., & Sarbatly, R. (2012). Conversion of microalgae to biofuel. Renewable and Sustainable Energy Reviews, 16(6), 4316–4342. https://doi.org/10.1016/j.rser.2012.03.047

Sutherland, D. L., Turnbull, M. H., & Craggs, R. J. (2014). Increased pond depth improves algal productivity and nutrient removal in wastewater treatment high rate algal ponds. Water Research, 53, 271–281. https://doi.org/10.1016/j.watres.2014.01.025

Uddin, M. N., Saha, G. C., Hasanath, M. A., Rahman, M. T., & Rashid, M. M. (2022). Development and Characterization of Novel Mn–Fe–Sn Ternary Nanoparticle by Sol–Gel Technique. In S. Arthur, M. Saitoh, & S. K. Pal (Eds.), Advances in Civil Engineering: Lecture Notes in Civil Engineering, Select Proceedings of ICACE 2020 (Vol. 184, pp. 45–54). Springer. https://doi.org/10.1007/978-981-16-5547-0_5

Umetani, I., Sposób, M., & Tiron, O. (2024). Semi-continuous cultivation for enhanced protein production using indigenous green microalgae and synthetic municipal wastewater. Journal of Applied Phycology, 36(3), 1105–1116. https://doi.org/10.1007/s10811-023-03179-6

Velásquez-Orta, S. B., Yáñez-Noguez, I., Ramírez, I. M., & Ledesma, M. T. O. (2024). Pilot-scale microalgae cultivation and wastewater treatment using high-rate ponds: a meta-analysis. Environmental Science and Pollution Research, 31(34), 46994–47021. https://doi.org/10.1007/s11356-024-34000-7

Villanova, V., Galasso, C., Vitale, G. A., Della Sala, G., Engelbrektsson, J., Strömberg, N., Shaikh, K. M., Andersson, M. X., Palma Esposito, F., Ekendahl, S., De Pascale, D., & Spetea, C. (2022). Mixotrophy in a Local Strain of Nannochloropsis granulata for Renewable High-Value Biomass Production on the West Coast of Sweden. Marine Drugs, 20(7), 424. https://doi.org/10.3390/md20070424

Yi, Z., Su, Y., Brynjolfsson, S., Olafsdóttir, K., & Fu, W. (2021). Bioactive polysaccharides and their derivatives from microalgae: biosynthesis, applications, and challenges, 71(1). https://doi.org/10.1016/B978-0-323-91095-8.00007-6

Downloads

Published

2026-07-22

How to Cite

Md Abul Hasanath, Md Mostafizur Rahman, Md Jahid Hasan, Md Nashir Uddin, Monir Hossain, Md Nazmul Hasan, … Nahid Islam. (2026). Integrated Nutrient Recovery and Decolorization from Textile Effluent in an Algal System: From Wastewater to Energy. Presidency University Journal, 4(1), 1–12. https://doi.org/10.66460/puj.v4i1.7

Issue

Section

Articles